PO0024 Integrating Complex Multi-Method Work?ows on a Single Hamilton?Microlab STAR?Workstation using INtip?Chemistries
來源:IMCS
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作者:Todd Mullis1, Michael Walla1, Jingyun Lee2, Cristina Furdui2, Casey Snodgrass3, Qian Wang1, L. Andrew Lee4*
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發布時間: 987天前
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944 次瀏覽
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INTRODUCTION
Automating complex, multi-step methods requires personnel with laboratory science,robotics programming skills, and design of experiment expertise. They must invest a signi?cant amount of time outlining the work?ow and transferring methods for robotic automation and obtaining such resources can be tasking. Here, we automated thecomplex phosphopeptide enrichment methods starting from crude cell lysate to liquidchromatography-mass spectrometry (LC-MS) ready processed samples as an example.
?Automated cell lysate processing
?Sequential sample processing for small number of samples (8+ samples) to larger number of samples
?Multi-step solid phase extraction processes using INtip chemistries
?Options and variability functions displayed on graphical user interface
?Modular pre-templated programs for protein digestion, peptide desalting and phosphopeptide enrichment run in single run or in three separate stages
閱讀原文
Automating complex, multi-step methods requires personnel with laboratory science,robotics programming skills, and design of experiment expertise. They must invest a signi?cant amount of time outlining the work?ow and transferring methods for robotic automation and obtaining such resources can be tasking. Here, we automated thecomplex phosphopeptide enrichment methods starting from crude cell lysate to liquidchromatography-mass spectrometry (LC-MS) ready processed samples as an example.
?Automated cell lysate processing
?Sequential sample processing for small number of samples (8+ samples) to larger number of samples
?Multi-step solid phase extraction processes using INtip chemistries
?Options and variability functions displayed on graphical user interface
?Modular pre-templated programs for protein digestion, peptide desalting and phosphopeptide enrichment run in single run or in three separate stages
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