PO0017 Dispersive Pipette Extraction for Automated Enrichment of IGF-1 from Serum
來源:IMCS
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作者:Sunil Hwang1, Todd Mullis1, Michael Mouradian2, L. Andrew Lee*1
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發布時間: 968天前
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953 次瀏覽
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Accurate and robust quanti?cation of insulin-like growth factor 1 (IGF-1) from serum using mass spectrometry (MS) is challenging due to its high complexity and dynamic range. Low cost,high throughput enrichment method for such low abundant proteins from the serum is required to enrich target proteins prior to MS analysis. We report a high-throughput sample reparation method using reverse phase micro-solid phase extraction enrichment on Microlab? NIMBUS? robotic liquid handling system from Hamilton Robotics and demonstrated over 71% recovery from serum for 96 samples in less than 30 minutes. IGF-1 levels in serum correlates well to other published methods that involve longer processing times.
INTRODUCTION
Automating complex, multi-step methods requires personnel with laboratory science,robotics programming skills, and design of experiment expertise. They must Insulin-like growth factor 1 (IGF-1) is a polypeptide hormone that plays an import role in childhood growth and continues to have anabolic e?ects in adults. Accurate and robust quanti?cation of IGF-1 from serum using mass spectrometry (MS) is challenging due to its high complexity and dynamic range. Low cost, high throughput enrichment method for such low abundant proteins from the serum is required to enrich target proteins prior to MS analysis.Herein, we report a high-throughput sample preparation method using reverse phase micro-solid phase extraction enrichment on a robotic liquid handling system.
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Automating complex, multi-step methods requires personnel with laboratory science,robotics programming skills, and design of experiment expertise. They must Insulin-like growth factor 1 (IGF-1) is a polypeptide hormone that plays an import role in childhood growth and continues to have anabolic e?ects in adults. Accurate and robust quanti?cation of IGF-1 from serum using mass spectrometry (MS) is challenging due to its high complexity and dynamic range. Low cost, high throughput enrichment method for such low abundant proteins from the serum is required to enrich target proteins prior to MS analysis.Herein, we report a high-throughput sample preparation method using reverse phase micro-solid phase extraction enrichment on a robotic liquid handling system.
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