PO0015 The Importance of pH in β-Glucuronidase Hydrolysis E?iciency
來源:MicroSep
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作者:P. Nikki Sitasuwan*1,
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發布時間: 969天前
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967 次瀏覽
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?Di?erent enzymes have di?erent optimal hydrolysis pH
?pH optima are substrate dependent
?Urine samples have a range of pH 4.0-9.0 and require bu?ers to adjust pH for the most e?cient hydrolysis
?Comparison of twoβ-glucuronidase hydrolysis e?ciencies at their optimal pH in both forti?ed synthetic urine and authentic patient urine samples
?pH optima are substrate dependent
?Urine samples have a range of pH 4.0-9.0 and require bu?ers to adjust pH for the most e?cient hydrolysis
?Comparison of twoβ-glucuronidase hydrolysis e?ciencies at their optimal pH in both forti?ed synthetic urine and authentic patient urine samples
β-glucuronidase is an enzyme commonly utilized in clinical and forensic labs to convertdrug metabolites present in biological ?uids into their parent compounds for betterdetection and quanti?cation. In the case of urine drug testing, urine samples are highlyheterogeneous and range from pH 4.6 to pH 8.0 (1), though pH may be outside this
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